Nutrition Research
Volume 23, Issue 10 , Pages 1313-1328 , October 2003

Effects of the consumption of Bifidobacterium lactis HN019 (DR10TM) and galacto-oligosaccharides on the microflora of the gastrointestinal tract in human subjects

  • Pramod K Gopal

      Affiliations

    • Bioscience Section, Fonterra Research Centre, Private Bag 11029, Dairy Farm Road, Palmerston North, New Zealand
    • Corresponding Author InformationCorresponding author. Tel.: +64-6-350-4600 ext 7062; fax: +64-6-350-4658.
    • ,
  • Jaya Prasad

      Affiliations

    • Bioscience Section, Fonterra Research Centre, Private Bag 11029, Dairy Farm Road, Palmerston North, New Zealand
    • ,
  • Harsharnjit S Gill

      Affiliations

    • Institute of Food, Nutrition and Human Health, Massey University, Palmerston North, New Zealand

Received 17 February 2003 ,Revised 1 June 2003 ,Accepted 11 June 2003.

References 

  1. Tannock GW. Role of probiotics. In:  Gibson GR,  Macfarlane GT editor. Human colonic bacteria: role in nutrition physiology and pathology. Florida: CRC Press; 1995;p. 257–271
  2. Gibson GR, Roberfroid MB. Dietary modulation of the human colonic microbiota (introducing concept of prebiotics). J Nutr. 1995;125:1401–1412
  3. Mitsuoka T. Intestinal flora and human health. Asia Pacific J Clin Nutr. 1996;5:2–5
  4. Rowland I. Metabolic interactions in the gut. In:  Fuller R editors. Probiotics: The scientific basis. London: Chapman & Hall; 1995;p. 29–53
  5. Mitsuoka T. Bifidobacteria and their role in human health. J Indust Microbiol. 1990;6:263–268
  6. Gibson GR. Dietary modulation of human gut microflora using prebiotics. Br J Nutr. 1998;80: S209–212
  7. Crittenden RG. Prebiotics. In:  Tannock GW editors. Probiotics (A critical review). Norfolk, England: Horizon Scientific Press; 1998;p. 141–157
  8. Bouhnik Y, Flourie B, Agay-Abensour D, Pochart P, Gramet G, Durand M, et al. Administration of transgalactooligosaccharides increases fecal bifidobacteria and modifies colonic fermentation metabolism in healthy humans. J Nutr. 1997;127:444–448
  9. Ito M, Deguchi Y, Miyamori A, Matsumoto K, Kikuchi H, Kobayashi Y, et al. Effects of administration of galactooligosaccharides on human faecal microflora, stool weight and abdominal sensation. Micro Eco Health Dis. 1990;3:285–292
  10. Prasad J, Gill HS, Smart J, Gopal PK. Selection and characterisation of lactobacillus and bifidobacterium strains for use as probiotics. Int Dairy Journal. 1998;8:993–1002
  11. Arunachalam K, Gill HS, Chandra RK. Enhancement of natural immune function by dietary consumption of B lactis HN019. Eur J Clinc Nutr. 2000;54:263–267
  12. Gill HS, Rutherfurd KJ, Cross ML. Dietary probiotic supplementation enhances natural killer cell activity in the elderly (an investigation of age-related immunological changes). J Clin Immunol. 2001;21:264–271
  13. Gill HS, Rutherfurd KJ, Cross ML, Gopal PK. Enhancement of immunity in the elderly by dietary supplementation with the probiotic Bifidobacterium lactis HN019. Am J Clin Nutr. 2001;74:833–839
  14. Gorbach SL, Chang T, Goldin B. Successful treatment of relapsing C. difficile colitis with Lactobacillus GG. Lancet. 1987;2:1519
  15. Schiffrin EJ, Rochat F, Link-Amster H, Aeschlimann JM, Donnet-Hughes A. Immunomodulation of human blood cells following the ingestion of lactic acid bacteria. J Dairy Sci. 1995;78:491–497
  16. Donnet-Hughes A, Rochat F, Link-Amster H, Aeschlimann JM, Schiffrin EJ. Modulation of nonspecific mechanisms of defense by lactic acid bacteria (effective dose). J Dairy Sci. 1999;82:863–869
  17. Bryant MP, Burkey LA. Cultural methods and some characteristics of the more numerous groups of bacteria in the bovine rumen. J Dairy Sci. 1953;36:205–209
  18. Sutter VL, Citron DM, Edelstein MAC, Finegold SM. Wadsworth anaerobic bacteriology manual. USA: Star Publishing Company; 1989;
  19. Beeren H. An elective and selective isolation medium for Bifidobacterium spp. Lett Appl Microbiol. 1990;11:155–157
  20. Rogosa M, Mitchell JA, Wiseman RF. A selective medium for the isolation of oral and faecal lactobacilli. J Bacteriol. 1951;62:132–133
  21. Barnes EM, Ingram M. The effect of redox potential on the grown C welchii strain isolated from horse muscle. J Appl Bacteriol. 1956;19:77–178
  22. Tannock GW, Munro K, Harmsen HJM, Welling GW, Smart J, Gopal PK. Analysis of the fecal microflora of human subjects consuming a probiotic product containing Lactobacillus rhamnosus DR20. J Appl Environ Microbiol. 2000;66:2578–2588
  23. Slanetz LW, Bartley CH. Numbers of enterococci in water, sewage and faeces determined by the membrane filter technique with an improved medium. J Bacteriol. 1957;74:591–595
  24. Kaufmann P, Pfefferkorn A, Teuber M, Meile L. Identification and quantification of Bifidobacterium species isolated from food with genus-specific 16S rRNA-targeted probes by colony hybridisation and PCR. Appl Environ Microbiol. 1997;63:1268–1273
  25. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning, a laboratory manual. 2nd ed. New York: Cold Spring Harbor Press; 1989;
  26. Turner KW, Thomas TD. Uncoupling of growth and acid production in lactic streptococci. NZ J Dairy Sci Technol. 1975;10:161–169
  27. Dubois M, Gilles K, Hamilton J, Robers P, Smith F. Colorimeteric method for determining sugars and related substances. Anal Chem. 1956;28:350–354
  28. Jackson P. High resolution polyacrylamide gel electrophoresis of fluorophore labelled reducing saccharides. Methods Enzymol. 1994;230:250–265
  29. Tannock GW. Medical importance of the normal microflora. Dordrecht/Boston/London: Kluwer Academic Publishers; 1999;
  30. Tannock GW. More than a smell (the complexity of normal microflora). In:  Tannock GW editors. Normal microflora: An introduction to microbes inhabiting the human body. London: Chapman & Hall; 1995;p. 24–28
  31. Gill HS. Stimulation of immune system by lactic cultures. Int Dairy J. 1998;8:535–544
  32. Romond M-E, Ais A, Guillemont F, Bounouader R, Cortot A, Romond C. Cell-free whey from milk fermented with Bifidobacterium breve C50 used to modify the colonic microflora of healthy subjects. J Dairy Sci. 1998;81:229–235
  33. Nielsen OH, Jorgensen S, Pedersen K, Justesen T. Microbiological evaluation of jejunal aspirates and faecal samples after oral administeration of bifidobacteria and lactic acid bacteria. J Appl Bacteriol. 1994;76:469–474
  34. Tanaka R, Takayama H, Mortomi M, Kuroshima T, Ueyama S, Matsumoto K, et al. Effects of administeration of TOS and Bifidobacterium breve 4006 on the human fecal flora. Bifidobacteria Microflora. 1983;2:17–24
  35. Ito M, Deguchi Y, Matsumoto K, Kimura M, Onodera N, Yajiama T. Influence of galactooligosaccharides on human fecal microflora. J Nutr Sci Vitaminol. 1993;39:635–640
  36. Alles M, Hartemink R, Meyboom S, Harryvan J, Katrien M, Nagengast F, et al. Effect of transgalactooligosaccharides on composition of the human intestinal microflora and on putative risk markers for colon cancer. Am J Clin Nutr. 1999;68:980–991
  37. Djouzi Z, Andrieux C. Compared effects of three oligosaccharides on metabolism of intestinal microflora in rats inoculated with a human faecal flora. Br J Nutr. 1997;78:313–324
  38. Rowland I, Tanaka R. The effect of transgalactosylated oligosaccharides on gut flora metabolism in rats associated with human faecal microflora. J Appl Bacteriol. 1993;74:667–674
  39. Gill H, Rutherfurd K, Prasad J, Gopal PK. Enhancement of natural and aquired immunity by Lactobacillus rhamnosus HN001, Lactobacillus acidophilus HN0017 and Bifidobacterium lactis HN019. Br J Nutr. 2000;83:167–176
  40. Benno Y, Mitsuaka T. Impact of Bifidobacterium longum on human fecal microflora. Microbiol Immunol. 1992;36:683–694
  41. Fukushima Y, Li S-T, Hara H, Terada A, Mitsuoka T. Effect of follow-up formula containing bifidobacteria (NAN BF) on fecal flora and fecal metabolites in healthy children. Bioscience Microflora. 1997;16:65–72
  42. Langhendries J, Detry J, Van Hees J, Lamboray J, Darimont J, Mozin M, et al. Effect of fermented infant formula containing vaible bifidobacteria on fecal flora composition and pH of healthy full-term infants. J Pediatr Gastroenterol Nutr. 1995;21:177–181
  43. Kullen MJ, Amann MM, O'Shaughnessy MJ, O'Sullivan DJ, Busta FF, Brady LJ. Differentiation of ingested and endogenous bifidobacteria by DNA fingerprinting demonstrates the survival of an unmodified strain in the gastrointestinal tract of humans. Am J Clin Nutr. 1997;64:89–94

PII: S0271-5317(03)00134-9

doi: 10.1016/S0271-5317(03)00134-9

Nutrition Research
Volume 23, Issue 10 , Pages 1313-1328 , October 2003

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