Incorporation of cis-9, trans-11 or trans-10, cis-12 conjugated linoleic acid into human erythrocytes in vivo

Burdge, Graham C.; Derrick, Peter R.; Russell, Jennifer J.; Tricon, Sabine; Kew, Samantha; Banerjee, Tapati; Grimble, Robert F.; Williams, Christine M.; Yaqoob, Parveen; Calder, Philip C.

doi:10.1016/j.nutres.2004.08.002

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Volume 25, Issue 1 , Pages 13-19, January 2005

Incorporation of cis-9, trans-11 or trans-10, cis-12 conjugated linoleic acid into human erythrocytes in vivo

Graham C. Burdge
- Institute of Human Nutrition, University of Southampton, Biomedical Sciences Building, Bassett Crescent East, Southampton, SO16 7PX, UK
- Corresponding author. Tel.: +44 02380 594315; fax: +44 02380 594379.
Peter R. Derrick
- Institute of Human Nutrition, University of Southampton, Biomedical Sciences Building, Bassett Crescent East, Southampton, SO16 7PX, UK
Jennifer J. Russell
- Institute of Human Nutrition, University of Southampton, Biomedical Sciences Building, Bassett Crescent East, Southampton, SO16 7PX, UK
Sabine Tricon
- Hugh Sinclair Unit for Human Nutrition, School of Food Biosciences, University of Reading, Reading, RG6 6AR, UK
Samantha Kew
- Hugh Sinclair Unit for Human Nutrition, School of Food Biosciences, University of Reading, Reading, RG6 6AR, UK
Tapati Banerjee
- Institute of Human Nutrition, University of Southampton, Biomedical Sciences Building, Bassett Crescent East, Southampton, SO16 7PX, UK
Robert F. Grimble
- Institute of Human Nutrition, University of Southampton, Biomedical Sciences Building, Bassett Crescent East, Southampton, SO16 7PX, UK
Christine M. Williams
- Hugh Sinclair Unit for Human Nutrition, School of Food Biosciences, University of Reading, Reading, RG6 6AR, UK
Parveen Yaqoob
- Hugh Sinclair Unit for Human Nutrition, School of Food Biosciences, University of Reading, Reading, RG6 6AR, UK
Philip C. Calder
- Institute of Human Nutrition, University of Southampton, Biomedical Sciences Building, Bassett Crescent East, Southampton, SO16 7PX, UK

Received 10 February 2004; received in revised form 13 August 2004; accepted 13 August 2004.

Abstract

The purpose of this study was to determine the incorporation into erythrocytes of cis (c)-9,trans (t)-11 conjugated linoleic acid (CLA) and t10,c12 CLA consumed as supplements highly enriched in these isomers. Healthy men (31 ± 8 years) consumed 1, 2, and 4 capsules containing approximately 80 g/100 g of either c9,t11 CLA or t10,c12 CLA for sequential 8-week periods. Fatty acid concentrations in erythrocyte total lipids were determined at baseline and after consumption of the highest dose. The increase in c9,t11 CLA concentration (0.31 g/100 g) was significantly greater than that in t10,c12 CLA (0.19 g/100 g). This was associated with minor changes in concentrations of some fatty acids of chain length greater than 20 carbons. These data suggest selective assimilation of individual CLA isomers into erythrocyte lipids and partial substitution for specific saturated and polyunsaturated fatty acids.

Keywords: Conjugated linoleic acid, human, erythrocyte